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Art for Mental Health

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Caleb Campbell
Caleb Campbell

50 Shades Of Gray Test.mov =LINK=


The movie adaptation of Fifty Shades Darker is scheduled to hit theaters on Feb. 10, continuing the risque storyline of the bestselling novel Fifty Shades of Grey series. Which got us wondering: Is it actually possible for a person to distinguish that many shades of such a neutral color?




50 shades of gray test.mov



What was Anastasia's major? How did Christian like to exercise? What was the name of his helicopter? From 514 pages and over two hours of film, can you remember all the shades or will you be left heartbroken before the sequel?


Cellular organization of brain tissue. In white matter (corona radiata), myelinated axons allow for rapid nerve impulse conduction; intermediate oligodendrocytes connect and form several myelin sheaths. Fibrous astrocytes ensure supply of nutrients and synaptic processing. In gray matter (cortex), neurons form synapses with each other and with protoplasmic astrocytes. In both white and gray matter, microglial cells contribute to clearance of debris and synapse remodeling. Reprinted from [27] under Creative Commons Attribution License (CC BY)


Figure 5 illustrates the major cell types in white and gray matter tissue. Gray matter regions contain mainly neuronal cell bodies, protoplasmic astrocytes providing neurons with nutrients, and microglia as active immune defense. White matter regions, on the contrary, contain axons, oligodendrocytes which wrap isolating myelin sheath around the axons, fibrous astrocytes, and microglia [27]. Notably, not only the cellular composition may be relevant for macroscopic tissue mechanics but also extracellular matrix components. The latter may show regional trends that even differ from those of brain cells.


Figure 10 shows stress relaxation experiments for all four brain regions [22], which confirm the extreme time-dependence of brain tissue with a stress relaxation of up to 80% within only 300 s. Interestingly, white matter tissue, with a stress relaxation of more than 70% after 300 s, is more viscous and responds more slowly than gray matter, with a stress relaxation of approximately 65%. Within white matter, specimens from the corpus callosum relax faster than specimens from the corona radiata. Within gray matter, the basal ganglia and the cortex exhibit a similar relaxation behavior. Stress relaxation percentages are slightly higher in shear than in compression, but both loading modes show similar regional dependencies.


Figures 11 and 12 illustrate that brain tissue not only stiffens with increasing strain but also with increasing strain rate. Rate-dependence of brain stiffness has consistently been reported in the literature using different testing techniques: shear testing or oscillatory shear testing [44, 127, 151], uniaxial compression or tension [57, 83, 85, 132, 137, 140], indentation [20, 107, 136, 163], and magnetic resonance elastography [36, 145]. Figure 11 illustrates uniaxial compression, tension, and simple shear experiments performed at strain rates of 0.33 and 0.0067 1/s, respectively. Figure 12 shows nanoindentation experiments over a loading rate spectrum from 1 to \(160\,\mu \hbox m/s\). Within the analyzed loading rate regime both gray and white matter double their maximum forces and corresponding moduli when increasing the loading rate by two orders of magnitude [20]. This effect becomes particularly important for applications such as blunt or traumatic brain injury, where even higher strain rates occur [137, 158].


Our results in Fig. 11 suggest that the effect of strain rate is more pronounced in white matter regions than in gray matter regions. This observation agrees well with the stress relaxation experiments in Fig. 10, which show that white matter relaxes faster than gray matter. A possible explanation for this behavior could be the difference in the permeability of gray and white matter. While for slower loadings, fluid has more time to escape, for faster loadings, it offers resistance, which leads to higher stresses. As white matter specimens seem to loose a higher amount of fluid during unconfined experiments than gray matter specimens [22, 23, 25], their strain rate effect is more pronounced.


Loading rate sensitivity of gray and white matter. Sensitivity of indentation force versus indentation depth for varying loading rates reveals the rate-dependent nature of brain tissue. Indentation force and modulus increase with increasing loading rate. Adapted from [20]


Our results agree well with an early study on the rheological shear response of human brain tissue [151], which showed higher directional variation in gray matter than in white matter, but neither of the differences appeared significant. Contradictory to our findings, studies on porcine brain tissue found a significantly stiffer shear response ortho